fish probes using the ribotm fluorescence in situ hybridization kit  (Ribobio co)

Journal: Experimental & Molecular Medicine

Article Title: LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver

doi: 10.1038/s12276-025-01420-5

Figure Lengend Snippet: The oligonucleotide sequences for siRNA experiments in vitro.

Article Snippet: Nucleic-acid-modified targeting Gm35585 oligonucleotide probes and RNA fluorescence in situ hybridization (FISH) kits (GenePharma) were used for RNA FISH of cell migration assays and tissue sections.

Techniques:

Journal: Experimental & Molecular Medicine

Article Title: LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver

doi: 10.1038/s12276-025-01420-5

Figure Lengend Snippet: Primers used for qRT–PCR analysis.

Article Snippet: Nucleic-acid-modified targeting Gm35585 oligonucleotide probes and RNA fluorescence in situ hybridization (FISH) kits (GenePharma) were used for RNA FISH of cell migration assays and tissue sections.

Techniques:

Journal: Experimental & Molecular Medicine

Article Title: LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver

doi: 10.1038/s12276-025-01420-5

Figure Lengend Snippet: a , f Costaining of liver tissue sections with Nile Red and EHHADH immunofluorescence. Scale bar, 25 μm. b , g Liver peroxidase activity. Reaction time: 30 min (mean ± s.d., n = 5, one-way ANOVA). c , h Liver total FA contents analyzed by LC–MS/MS (mean ± s.d., n = 5, one-way ANOVA). d , i Heat map of 28 FA contents in the liver. (* P <0.05, ** P <0.01, *** P <0.001, NCD+AAV-NC vs HFD+AAV-NC, and NCD+TBG-NC vs HFD+TBG-NC. # P <0.05, ## P <0.01, ### P <0.001, HFD+AAV-NC vs HFD+AAVGm35585, and HFD+TBG-NC vs HFD+TBG-EHHADH.) e , j Changes in hepatic SCFA, MCFA and LCFA levels. Information on the 28 FAs is given in Table . ( a – e The analysis of Gm35585 overexpression experiments in vivo. f – j The analysis of EHHADH overexpression experiments in vivo.).

Article Snippet: Nucleic-acid-modified targeting Gm35585 oligonucleotide probes and RNA fluorescence in situ hybridization (FISH) kits (GenePharma) were used for RNA FISH of cell migration assays and tissue sections.

Techniques: Immunofluorescence, Activity Assay, Liquid Chromatography with Mass Spectroscopy, Over Expression, In Vivo

Journal: Experimental & Molecular Medicine

Article Title: LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver

doi: 10.1038/s12276-025-01420-5

Figure Lengend Snippet: a In vivo experimental flow diagram. NCD mice: C57BL/6J mice; HFD mice: 10 weeks of high-fat feeding C57BL/6J mice. b Changes in the body weight of mice within 10 days after AAV-Gm35585 injection (mean ± s.d., n = 5, one-way ANOVA; **** P < 0.0001, NCD + AAV-NC versus HFD + AAV-NC; # P < 0.05, ## P < 0.01, ### P < 0.001, HFD + AAV-NC versus HFD + AAV-Gm35585). c Changes in liver TG and TC levels under Gm35585 overexpression condition (mean ± s.d., n = 5; one-way ANOVA). d Serum biochemical indices included ALT, AST, TG, TC, LDL, and HDL (mean ± s.d., n = 5; one-way ANOVA). e Oil Red O staining of liver tissue sections indicating changes in lipid droplet content. Scale bar, 50 μm. f Differences between groups after Gm35585 overexpression ( n = 3). g The nnumber of upregulated and downregulated genes after Gm35585 overexpression ( n = 3). h KEGG enrichment analysis of RNA sequencing data from HFD + AVV-Gm35585 mouse livers.

Article Snippet: Nucleic-acid-modified targeting Gm35585 oligonucleotide probes and RNA fluorescence in situ hybridization (FISH) kits (GenePharma) were used for RNA FISH of cell migration assays and tissue sections.

Techniques: In Vivo, Injection, Over Expression, Staining, RNA Sequencing

Journal: Experimental & Molecular Medicine

Article Title: LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver

doi: 10.1038/s12276-025-01420-5

Figure Lengend Snippet: a , f Costaining of liver tissue sections with Nile Red and EHHADH immunofluorescence. Scale bar, 25 μm. b , g Liver peroxidase activity. Reaction time: 30 min (mean ± s.d., n = 5, one-way ANOVA). c , h Liver total FA contents analyzed by LC–MS/MS (mean ± s.d., n = 5, one-way ANOVA). d , i Heat map of 28 FA contents in the liver. * P < 0.05, ** P < 0.01, *** P < 0.001, NCD+AAV-NC vs HFD+AAV-NC, and NCD+TBG-NC vs HFD+TBG-NC. # P < 0.05, ## P < 0.01, ### P < 0.001, HFD+AAV-NC vs HFD+AAV-Gm35585, and HFD+TBG-NC vs HFD+TBG-EHHADH. e , j Changes in hepatic SCFA, MCFA and LCFA levels. Information on the 28 FAs is given in Table . ( a – e The analysis of Gm35585 overexpression experiments in vivo. f – j The analysis of EHHADH overexpression experiments in vivo).

Article Snippet: Nucleic-acid-modified targeting Gm35585 oligonucleotide probes and RNA fluorescence in situ hybridization (FISH) kits (GenePharma) were used for RNA FISH of cell migration assays and tissue sections.

Techniques: Immunofluorescence, Activity Assay, Liquid Chromatography with Mass Spectroscopy, Over Expression, In Vivo

Journal: Experimental & Molecular Medicine

Article Title: LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver

doi: 10.1038/s12276-025-01420-5

Figure Lengend Snippet: a mRNA expression levels of Gm35585, Pparα and Ehhadh with PA and siGm35585 treatment in AML-12 cells. The values were normalized to those of the control group (mean ± s.d., n = 3, one-way ANOVA). b , j , n The protein expression of PPARα and EHHADH in AML-12 cells. c , g , k , o Nile Red staining of AML-12 cells to test changes in lipid content. Scale bar, 50 μm. d , h , l , p Changes in TG levels in AML-12 cells (mean ± s.d., n = 3, one-way ANOVA). e mRNA expression levels of Ehhadh with PA, siEHHADH and pEX-3-Gm35585 treatment in AML-12 cells. The values were normalized to those of the control group (mean ± s.d., n = 3, one-way ANOVA). f The protein expression of EHHADH in AML-12 cells. i mRNA expression levels of Pparα and Ehhadh with PA and GW7647 treatment in AML-12 cells. The values were normalized to those of the control group (mean ± s.d., n = 3, one-way ANOVA). m mRNA expression levels of Pparα and Ehhadh with PA, GW6471 and pEX-3-Gm35585 treatment in AML-12 cells. The values were normalized to those of the control group (mean ± s.d., n = 3, one-way ANOVA). ( b – d The analysis in AML-12 cells with PA and siGm35585 treatment. g , h The analysis in AML-12 cells with PA, siEHHADH, and pEX-3-Gm35585 treatment. j – l The analysis in AML-12 cells with PA, siEHHADH, and GW7646 treatment. n – p The analysis in AML-12 cells with PA, GW6471, and pEX-3-Gm35585 treatment.).

Article Snippet: Nucleic-acid-modified targeting Gm35585 oligonucleotide probes and RNA fluorescence in situ hybridization (FISH) kits (GenePharma) were used for RNA FISH of cell migration assays and tissue sections.

Techniques: Expressing, Control, Staining

Journal: Experimental & Molecular Medicine

Article Title: LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver

doi: 10.1038/s12276-025-01420-5

Figure Lengend Snippet: a Gm35585 RNA FISH and EHHADH immunofluorescence costaining under PA and pEX-3-Gm35585 treatment in AML-12 cells. Scale bar, 25 μm. b Gm35585 expression levels in the nucleus and cytoplasm of AML-12 cells. The values were normalized to those of the control group (mean ± s.d., n = 3, two-way ANOVA). c Gm35585 expression in the nucleus and cytoplasm in the liver. The values were normalized to those of the control group (mean ± s.d., n = 3, two-way ANOVA). d RNA pulldown assay flowchart in AML-12 cells. e Silver staining followed by an RNA pulldown assay in AML-12 cells. A U-biotin-labeled Gm35585 probe was used to pull down proteins. f KEGG enrichment analysis of the RNA pulldown protein profile. g Protein expression levels of RXRα and RXRβ in Gm35585 overexpression mouse liver. h mRNA expression levels of Rxrα and Rxrβ in Gm35585 overexpression mouse liver. The values were normalized to those of the control group (mean ± s.d., n = 5; one-way ANOVA). i Protein expression levels of RXRα and RXRβ in AML-12 cells. j mRNA expression levels of Rxrα and Rxrβ in AML-12 cells. The values were normalized to those of the control group (mean ± s.d., n = 4, one-way ANOVA). k Western blot analysis of RNA pulldown proteins. l Mechanistic diagram of Gm35585 binding to RXRα.

Article Snippet: Nucleic-acid-modified targeting Gm35585 oligonucleotide probes and RNA fluorescence in situ hybridization (FISH) kits (GenePharma) were used for RNA FISH of cell migration assays and tissue sections.

Techniques: Immunofluorescence, Expressing, Control, Silver Staining, Labeling, Over Expression, Western Blot, Binding Assay

Journal: Experimental & Molecular Medicine

Article Title: LncRNA Gm35585 transcriptionally activates the peroxidase EHHADH against diet-induced fatty liver

doi: 10.1038/s12276-025-01420-5

Figure Lengend Snippet: a Western blotting assay followed by co-IP assay under IgG and RXRα antibody treatment in AML-12 cells. b The JASPAR database shows the sequence of the PPARα/RXRα transcription factor DAN, which binds to the EHHADH promoter. c The position of the ChIP primer in the EHHADH promoter; the red font represents a DNA sequence similar to that in ( b ). d A ChIP assay combined with RT‒qPCR was performed using the IgG and RXRα antibodies in AML-12 cells (mean ± s.d., n = 4, t test). e mRNA expression levels of Rxrα and Ehhadh with PA, siRXRα and pEX-3-Gm35585 treatment in AML-12 cells. The values were normalized to those of the control group (mean ± s.d., n = 4, one-way ANOVA). f Protein expression levels of RXRα and EHHADH in AML-12 cells. g Changes in TG levels in AML-12 cells (mean ± s.d., n = 4, one-way ANOVA). h Nile Red and EHHADH immunofluorescence costaining in AML-12 cells. Scale bar, 25 μm. i Total mechanism chart.

Article Snippet: Nucleic-acid-modified targeting Gm35585 oligonucleotide probes and RNA fluorescence in situ hybridization (FISH) kits (GenePharma) were used for RNA FISH of cell migration assays and tissue sections.

Techniques: Western Blot, Co-Immunoprecipitation Assay, Sequencing, Expressing, Control, Immunofluorescence

Journal: Oncology Letters

Article Title: Coexistence of acute severe leukocytosis and anaplastic lymphoma kinase‑positive histiocytic sarcoma, a rare entity with an unusual presentation: A case report

doi: 10.3892/ol.2024.14649

Figure Lengend Snippet: EML4/ALK. Four gene regions were analyzed, 24/100 cells were 2p23 translocated and the cut off was 15%.

Article Snippet: Translocation of EML4/ALK 2p23 was assessed using the Vysis ALK break-apart fluorescence in situ hybridization (FISH) probe kit (cat. no. 30-608916/R8; Abbott Laboratories) ( ).

Techniques:

Journal: Cancers

Article Title: Optimal Treatment Strategy for Oligo-Recurrence Lung Cancer Patients with Driver Mutations

doi: 10.3390/cancers16020464

Figure Lengend Snippet: Study design and flow chart of this study. LAT, local ablative therapy; MTT, molecular targeted therapy; IQR, interquartile range; EGFR, epidermal growth factor receptor; ALK, anaplastic lymphoma kinase.

Article Snippet: Positive cases were further examined for confirmation using Vysis ® ALK Break-Apart fluorescence in situ hybridization (FISH) Probe Kits (Abbott Molecular, Chicago, IL, USA).

Techniques: